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| Registros recuperados: 12 | |
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| Fernando,Juliana Aparecida; Vieira,Maria Lúcia Carneiro; Geraldi,Isaías Olívio; Appezzato-da-Glória,Beatriz. |
| A comparative anatomical analysis of somatic embryogenesis in two soybean (Glycine max (L.) Merrill) genotypes was carried out. The somatic embryos were originated from cotyledonary explants obtained from immature zygotic embryos. The medium used for somatic embryogenesis induction was Murashige and Skoog, 1962, salts and Gamborg et al., 1968, vitamins (MSB) supplemented with 0.8 mg.L-1 of 2,4-D for genotype PI 123439 and 40 mg.L-1 of 2,4-D for Williams 82. Globular structures, constituted by meristematic cells, originated from subepidermal cell divisions of the cotyledonary mesophyll. In PI 123439, the globular structures presented tracheary differentiation among meristematic cells and they could follow distinct morphogenetic process depending on their... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max anatomy somatic embryogenesis 2; 4-D. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300005 |
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| Glienke-Blanco,Chirlei; Aguilar-Vildoso,Carlos Ivan; Vieira,Maria Lúcia Carneiro; Barroso,Paulo Augusto Vianna; Azevedo,João Lúcio. |
| During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Endophytes; Genetic variability; Guignardia citricarpa; Mandarin; RAPD. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200021 |
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| Mondin,Mateus; Oliveira,Carlos Alberto de; Vieira,Maria Lúcia Carneiro. |
| Malpighia emarginata Sessé & Mociño ex DC. or West Indian cherry (acerola) is a wild plant originated in southern Mexico, Central America and the northern region of South America. The species was introduced to Brazil about 60 years ago and now the country is the world's biggest producer. Even though the fruits of acerola have high commercial value, as they are an important source of the natural vitamin C, very little chromosome information is available for this species. Previous studies showed that most Malpighia species are diploids, including M. emarginata with 2n = 20. In the present paper, the chromosome number of acerola was confirmed, and for the first time, its karyotype was described, providing the identification of the homologues for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acerola; Cytology; Chromosomes; In vitro culture establishment; West Indian cherry. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200005 |
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| Oliveira,Eder Jorge; Pádua,Juliano Gomes; Zucchi,Maria Imaculada; Vencovsky,Roland; Vieira,Maria Lúcia Carneiro. |
| Microsatellites, or simple sequence repeats (SSRs), have been the most widely applied class of molecular markers used in genetic studies, with applications in many fields of genetics including genetic conservation, population genetics, molecular breeding, and paternity testing. This range of applications is due to the fact that microsatellite markers are co-dominant and multi-allelic, are highly reproducible, have high-resolution and are based on the polymerase chain reaction (PCR). When first introduced, the development of microsatellite markers was expensive but now new and efficient methods of repetitive sequence isolation have been reported, which have led to reduced costs and microsatellite-technology has been increasingly applied to several species,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellites; Molecular genetics; Genetic structure of populations. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200018 |
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| Barroso,Paulo Augusto Vianna; Geraldi,Isaias Olívio; Vieira,Maria Lúcia Carneiro; Pulcinelli,Carlos Eduardo; Vencovsky,Roland; Dias,Carlos Tadeu dos Santos. |
| In order to verify whether genetic distance (GD) is associated with population mean (PM), genetic variance (GV) and the proportion of superior progenies generated by each cross in advanced generations of selfing (PS), the genetic distances between eight soybean lines (five adapted and three non-adapted) were estimated using 213 polymorphic RAPD markers. The genetic distances were partitioned according to Griffing's Model I Method 4 for diallel analysis, i.e., GDij = GD+ GGDi+ GGDj + SGDij. Phenotypic data were recorded for seed yield and plant height for 25 out of 28 populations of a diallel set derived from the eight soybean lines and evaluated from F2:8 to F2:11 generations. No significant correlation for seed yield was detected between GD and GV, while... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soybean; Genetic distance; Molecular markers; RAPD; Prediction. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300020 |
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| Cuco,Silvia Marina; Mondin,Mateus; Vieira,Maria Lúcia Carneiro; Aguiar-Perecin,Margarida L. R.. |
| Foram conduzidos experimentos visando à otimização de procedimentos para preparações citológicas de pontas de raiz com alta freqüência de metáfases mitóticas, apresentando cromossomos com morfologia nítida, em espécies pertencentes a dois gêneros de plantas, Passiflora (Passifloraceae) e Crotalaria (Leguminosae). Para o acúmulo de metáfases, bem como de prófases e prometáfases com cromossomos bem condensados, foram avaliados pré-tratamentos de raízes, em que foram utilizadas diferentes combinações de 8-hidroxiquinolina, inibidor do fuso mitótico, com cicloheximida, inibidor da síntese protéica. As preparações foram coradas pelo método de Feulgen. Os melhores resultados foram obtidos com o tratamento com 8-hidroxiquinolina a 300ppm combinado com... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passiflora; Crotalaria; Citogenética vegetal; Cromossomos mitóticos; 8-hidroxiquinolina; Cicloheximida. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062003000300004 |
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| Registros recuperados: 12 | |
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